Chemical fingerprinting of three Anemone species and an adulteration study to detect cross mixing of medicinal plants by HPLC-HR-ESI-MS/MS method

The adulteration of plant raw materials used for the preparation herbal drugs with foreign material is one important issues in quality control products. Chemical fingerprinting a well-known approach characterization secondary metabolites associated species and can be material. current study centred on development chemical three medicinal plants genus Anemone including A. obtusiloba, falconeri A tetrasepala through identification their using LC-ESI-QTOF-MS/MS analysis. Thirty compounds were identified by high-resolution positive negative electrospray- ionization (ESI) modes MS/MS belong to diterpenoids, alkaloids, phenols, flavonoids other classes distribution among analysed was studied different statistical tools. Moreover, an LC-HR-ESI-MS/MS method developed detect cross mixing obtusiloba Ziziphus jujuba. Seven chromatographically differentiative peaks confined selected its contamination adulterated samples. able as low 20% Z. This play significant role manage medicines identify lead natural products these plants.